› 6nfj

X-ray diffraction
3.19Å resolution

Structure of Beta-Klotho in Complex with FGF19 C-terminal peptide

Released:
DOI: 10.2210/pdb6nfj/pdb
PDB entry 6nfj coloured by chain, front view.
PDB entry 6nfj coloured by chain, side view.
PDB entry 6nfj coloured by chain, top view.
Source organisms:
Primary publication:
Structures of ligand-occupied β-Klotho complexes reveal a molecular mechanism underlying endocrine FGF specificity and activity.
Kuzina ES, Ung PM, Mohanty J, Tome F, Choi J, Pardon E, Steyaert J, Lax I, Schlessinger A, Schlessinger J, Lee S
Proc Natl Acad Sci U S A 116 7819-7824 (2019)
PMID: 30944224

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-231678 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-klotho Chains: A, D
Molecule details ›
Chains: A, D
Length: 960 amino acids
Theoretical weight: 110.39 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q86Z14 (Residues: 30-984; Coverage: 92%)
Gene name: KLB
Sequence domains: Glycosyl hydrolase family 1
Nanobody 30 Chains: B, E
Molecule details ›
Chains: B, E
Length: 133 amino acids
Theoretical weight: 14.7 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Fibroblast growth factor 19 Chains: C, F
Molecule details ›
Chains: C, F
Length: 50 amino acids
Theoretical weight: 5.55 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O95750 (Residues: 167-216; Coverage: 26%)
Gene names: FGF19, UNQ334/PRO533

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 130.09Å b: 143.9Å c: 141.05Å
α: 90° β: 90.9° γ: 90°
R-values:
R R work R free
0.282 0.28 0.32
Expression systems:
  • Homo sapiens
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

Receptor tyrosine kinase activation: From the ligand perspective.
Trenker et al. (2020)
10 more

2 mentions without citation

Structures of ligand-occupied β-Klotho complexes reveal a molecular mechanism underlying endocrine FGF specificity and activity.
Kuzina et al. (2019)
1 more