› 6ng0

X-ray diffraction
2.05Å resolution

Crystal structure of HPK1 kinase domain T165E,S171E phosphomimetic mutant in complex with sunitinib in the inactive state.

Released:
DOI: 10.2210/pdb6ng0/pdb
PDB entry 6ng0 coloured by chain, front view.
PDB entry 6ng0 coloured by chain, side view.
PDB entry 6ng0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Multiple conformational states of the HPK1 kinase domain in complex with sunitinib reveal the structural changes accompanying HPK1 trans-regulation.
Johnson E, McTigue M, Gallego RA, Johnson TW, Timofeevski S, Maestre M, Fisher TS, Kania R, Sawasdikosol S, Burakoff S, Cronin CN
J Biol Chem 294 9029-9036 (2019)
PMID: 31018963

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187814 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase kinase kinase kinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 34.5 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q92918 (Residues: 1-307; Coverage: 37%)
Gene names: HPK1, MAP4K1
Sequence domains: Protein kinase domain

Ligands and Environments

1 bound ligand:
Ligand B49 2 x B49
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P1
Unit cell:
a: 55.81Å b: 58.92Å c: 60.93Å
α: 82.44° β: 82.31° γ: 64.34°
R-values:
R R work R free
0.202 0.201 0.229
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

A perspective on HPK1 as a novel immuno-oncology drug target.
Sawasdikosol et al. (2020)
8 other articles

3 mentions without citation

Multiple conformational states of the HPK1 kinase domain in complex with sunitinib reveal the structural changes accompanying HPK1 trans-regulation.
Johnson et al. (2019)
2 more