› 6ni2

Electron Microscopy
4Å resolution

Stabilized beta-arrestin 1-V2T subcomplex of a GPCR-G protein-beta-arrestin mega-complex

Released:
DOI: 10.2210/pdb6ni2/pdb
PDB entry 6ni2 coloured by chain, front view.
PDB entry 6ni2 coloured by chain, side view.
PDB entry 6ni2 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of an endosomal signaling GPCR-G protein-β-arrestin megacomplex.
Nguyen AH, Thomsen ARB, Cahill TJ, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Little J, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ
Nat Struct Mol Biol 26 1123-1131 (2019)
PMID: 31740855
Related structures: EMD-9375

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-210713 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Nanobody 32 Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.67 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Beta-arrestin-1 Chain: B
Molecule details ›
Chain: B
Length: 393 amino acids
Theoretical weight: 44.31 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P17870 (Residues: 1-393; Coverage: 94%)
Gene name: ARRB1
Sequence domains:
Fab30 Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 237 amino acids
Theoretical weight: 25.51 KDa
Source organism: synthetic construct
Expression system: Not provided
Fab30 Light Chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.44 KDa
Source organism: synthetic construct
Expression system: Not provided
Vasopressin V2 receptor Chain: V
Molecule details ›
Chain: V
Length: 26 amino acids
Theoretical weight: 3.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P30518 (Residues: 343-368; Coverage: 7%)
Gene names: ADHR, AVPR2, DIR, DIR3, V2R

Ligands and Environments

No bound ligands
2 modified residues:
Ligand SEP 4 x SEP
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-9375
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

26 review citations

Biased GPCR signaling: Possible mechanisms and inherent limitations.
Gurevich et al. (2020)
25 more

7 mentions without citation

Differential Regulation of GPCRs-Are GRK Expression Levels the Key?
Matthees et al. (2021)
6 more