› 6ni3

Electron Microscopy
3.8Å resolution

B2V2R-Gs protein subcomplex of a GPCR-G protein-beta-arrestin mega-complex

Released:
DOI: 10.2210/pdb6ni3/pdb
PDB entry 6ni3 coloured by chain, front view.
PDB entry 6ni3 coloured by chain, side view.
PDB entry 6ni3 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of an endosomal signaling GPCR-G protein-β-arrestin megacomplex.
Nguyen AH, Thomsen ARB, Cahill TJ, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Little J, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ
Nat Struct Mol Biol 26 1123-1131 (2019)
PMID: 31740855
Related structures: EMD-9376

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-207238 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.33 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 96%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.53 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Beta-2 adrenergic receptor; Endolysin Chain: R
Molecule details ›
Chain: R
Length: 506 amino acids
Theoretical weight: 57.63 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0A097J809 (Residues: 2-161; Coverage: 98%)
  • Canonical: P07550 (Residues: 29-341; Coverage: 76%)
Gene names: ADRB2, ADRB2R, B2AR, RB59_126, e
Sequence domains:
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 138 amino acids
Theoretical weight: 15.14 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand P0G 1 x P0G
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-9376
Expression systems:
  • Trichoplusia ni
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

27 review citations

Biased GPCR signaling: Possible mechanisms and inherent limitations.
Gurevich et al. (2020)
26 more

7 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
6 more