› 6niy

Electron Microscopy
3.34Å resolution

A high-resolution cryo-electron microscopy structure of a calcitonin receptor-heterotrimeric Gs protein complex

Released:
DOI: 10.2210/pdb6niy/pdb
PDB entry 6niy coloured by chain, front view.
PDB entry 6niy coloured by chain, side view.
PDB entry 6niy coloured by chain, top view.
Source organisms:
Primary publication:
The Molecular Control of Calcitonin Receptor Signaling.
Dal Maso E, Glukhova A, Zhu Y, Garcia-Nafria J, Tate CG, Atanasio S, Reynolds CA, Ramírez-Aportela E, Carazo JM, Hick CA, Furness SGB, Hay DL, Liang YL, Miller LJ, Christopoulos A, Wang MW, Wootten D, Sexton PM
ACS Pharmacol Transl Sci 2 31-51 (2019)
PMID: 32219215
Related structures: EMD-9382

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-211024 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.73 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Nanobody35 Chain: N
Molecule details ›
Chain: N
Length: 138 amino acids
Theoretical weight: 15.14 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Calcitonin receptor Chain: R
Molecule details ›
Chain: R
Length: 474 amino acids
Theoretical weight: 55.39 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P30988 (Residues: 1-474; Coverage: 100%)
Gene name: CALCR
Sequence domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Structure domains: Transducin (heterotrimeric G protein), gamma chain
Calcitonin peptide-like domain-containing protein Chain: P
Molecule details ›
Chain: P
Length: 32 amino acids
Theoretical weight: 3.44 KDa
Source organism: Oncorhynchus sp.
Expression system: Not provided
UniProt:
  • Canonical: Q92163 (Residues: 9-40; Coverage: 52%)
Gene name: CGRP
Sequence domains: Calcitonin / CGRP / IAPP family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.34Å
Relevant EMDB volumes: EMD-9382
Expression systems:
  • Trichoplusia ni
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Capturing Peptide-GPCR Interactions and Their Dynamics.
Kaiser et al. (2020)
4 more

9 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
8 more