› 6nkf

X-ray diffraction
2.23Å resolution

Crystal Structure of the Lipase Lip_vut4 from Goat Rumen metagenome.

Released:
DOI: 10.2210/pdb6nkf/pdb
PDB entry 6nkf coloured by chain, front view.
PDB entry 6nkf coloured by chain, side view.
PDB entry 6nkf coloured by chain, top view.
Source organism: metagenome
Entry authors: Kim Y, Welk L, Mukendi G, Nkhi G, Motloi T, Jedrzejczak R, Feto N, Joachimiak A

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179205 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BD-FAE-like domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 304 amino acids
Theoretical weight: 34.47 KDa
Source organism: metagenome
Expression system: Escherichia coli
UniProt:
  • Canonical: Q65FG3 (Residues: 1-298; Coverage: 100%)
Gene name: BL02554
Sequence domains: BD-FAE

Ligands and Environments

3 bound ligands:
Ligand EDO 22 x EDO
Ligand PEG 3 x PEG
Ligand SBT 1 x SBT
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 157.856Å b: 157.856Å c: 195.492Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.154 0.179
Expression system: Escherichia coli

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