› 6nqj

X-ray diffraction
2Å resolution

Crystal structure of fast switching M159T mutant of fluorescent protein Dronpa (Dronpa2)

Released:
DOI: 10.2210/pdb6nqj/pdb
PDB entry 6nqj coloured by chain, front view.
PDB entry 6nqj coloured by chain, side view.
PDB entry 6nqj coloured by chain, top view.
Source organism: Echinophyllia sp. SC22
Primary publication:
Electrostatic control of photoisomerization pathways in proteins.
Romei MG, Lin CY, Mathews II, Boxer SG
Science 367 76-79 (2020)
PMID: 31896714

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178341 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 255 amino acids
Theoretical weight: 28.96 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5TLG6 (Residues: 3-223; Coverage: 99%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GYC 4 x GYC

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 70.03Å b: 85.795Å c: 78.582Å
α: 90° β: 110.11° γ: 90°
R-values:
R R work R free
0.177 0.174 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Photoswitchable Fluorescent Proteins: Mechanisms on Ultrafast Timescales.
Tang et al. (2022)
3 more

4 mentions without citation

Electrostatic control of photoisomerization pathways in proteins.
Romei et al. (2020)
3 more