› 6nyp

X-ray diffraction
2.7Å resolution

Crystal structure of UL144/BTLA complex

Released:
DOI: 10.2210/pdb6nyp/pdb
PDB entry 6nyp coloured by chain, front view.
PDB entry 6nyp coloured by chain, side view.
PDB entry 6nyp coloured by chain, top view.
Source organisms:
Primary publication:
Structure of human cytomegalovirus UL144, an HVEM orthologue, bound to the B and T cell lymphocyte attenuator.
Bitra A, Nemčovičová I, Picarda G, Doukov T, Wang J, Benedict CA, Zajonc DM
J Biol Chem 294 10519-10529 (2019)
PMID: 31126984

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167169 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
B- and T-lymphocyte attenuator Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 107 amino acids
Theoretical weight: 12.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z6A9 (Residues: 31-137; Coverage: 41%)
Gene name: BTLA
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins
UL144 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 118 amino acids
Theoretical weight: 13.19 KDa
Source organism: Macacine alphaherpesvirus 1
Expression system: Spodoptera frugiperda

Ligands and Environments

4 bound ligands:
Ligand GOL 1 x GOL
Ligand SO4 5 x SO4
Ligand NA 2 x NA
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 66.99Å b: 77.2Å c: 101.72Å
α: 90° β: 91.67° γ: 90°
R-values:
R R work R free
0.232 0.23 0.276
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

2 review citations

Immune Checkpoints, a Novel Class of Therapeutic Targets for Autoimmune Diseases.
Zhai et al. (2021)
1 more

2 mentions without citation

Structure of human cytomegalovirus UL144, an HVEM orthologue, bound to the B and T cell lymphocyte attenuator.
Bitra et al. (2019)
1 more