› 6o0t

X-ray diffraction
2.8Å resolution

Crystal structure of selenomethionine labelled tandem SAM domains (L446M:L505M:L523M mutant) from human SARM1

Released:
DOI: 10.2210/pdb6o0t/pdb
PDB entry 6o0t coloured by chain, front view.
PDB entry 6o0t coloured by chain, side view.
PDB entry 6o0t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
NAD+ cleavage activity by animal and plant TIR domains in cell death pathways.
Horsefield S, Burdett H, Zhang X, Manik MK, Shi Y, Chen J, Qi T, Gilley J, Lai JS, Rank MX, Casey LW, Gu W, Ericsson DJ, Foley G, Hughes RO, Bosanac T, von Itzstein M, Rathjen JP, Nanson JD, Boden M, Dry IB, Williams SJ, Staskawicz BJ, Coleman MP, Ve T, Dodds PN, Kobe B
Science 365 793-799 (2019)
PMID: 31439792

Function and Biology Details

Reaction catalysed: 
(1a) NAD(+) = cyclic ADP-ribose + nicotinamide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180347 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(+) hydrolase SARM1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 177 amino acids
Theoretical weight: 20.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6SZW1 (Residues: 409-561; Coverage: 21%)
Gene names: KIAA0524, SAMD2, SARM, SARM1
Sequence domains: SAM domain (Sterile alpha motif)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Unit cell:
a: 94.24Å b: 154.08Å c: 120.995Å
α: 90° β: 94.55° γ: 90°
R-values:
R R work R free
0.202 0.2 0.238
Expression system: Escherichia coli

Quick links

Citations

51 review citations

NOD-like receptor-mediated plant immunity: from structure to cell death.
Saur et al. (2021)
50 more