› 6o0z

Electron Microscopy
3.3Å resolution

Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium

Released:
DOI: 10.2210/pdb6o0z/pdb
PDB entry 6o0z coloured by chain, front view.
PDB entry 6o0z coloured by chain, side view.
PDB entry 6o0z coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9.
Zhu X, Clarke R, Puppala AK, Chittori S, Merk A, Merrill BJ, Simonović M, Subramaniam S
Nat Struct Mol Biol 26 679-685 (2019)
PMID: 31285607
Related structures: EMD-0585

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120360 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: A
Molecule details ›
Chain: A
Length: 1372 amino acids
Theoretical weight: 158.99 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
target strand DNA Chain: C
Molecule details ›
Chain: C
Length: 40 nucleotides
Theoretical weight: 12.18 KDa
Source organism: synthetic construct
Expression system: Not provided
non-target strand DNA Chain: D
Molecule details ›
Chain: D
Length: 40 nucleotides
Theoretical weight: 12.45 KDa
Source organism: synthetic construct
Expression system: Not provided
single guide RNA Chain: B
Molecule details ›
Chain: B
Length: 102 nucleotides
Theoretical weight: 33.06 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-0585
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

CRISPR/Cas9 therapeutics: progress and prospects.
Li et al. (2023)
10 more

10 mentions without citation

Twisting and swiveling domain motions in Cas9 to recognize target DNA duplexes, make double-strand breaks, and release cleaved duplexes.
Wang et al. (2022)
9 more