› 6o81

Electron Microscopy
3.21Å resolution

Electron cryo-microscopy of the eukaryotic translation initiation factor 2B bound to translation initiation factor 2 from Homo sapiens

Released:
DOI: 10.2210/pdb6o81/pdb
PDB entry 6o81 coloured by chain, front view.
PDB entry 6o81 coloured by chain, side view.
PDB entry 6o81 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
eIF2B-catalyzed nucleotide exchange and phosphoregulation by the integrated stress response.
Kenner LR, Anand AA, Nguyen HC, Myasnikov AG, Klose CJ, McGeever LA, Tsai JC, Miller-Vedam LE, Walter P, Frost A
Science 364 491-495 (2019)
PMID: 31048491
Related structures: EMD-0649

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138592 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Translation initiation factor eIF2B subunit epsilon Chains: A, B
Molecule details ›
Chains: A, B
Length: 721 amino acids
Theoretical weight: 80.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13144 (Residues: 1-721; Coverage: 100%)
Gene names: EIF2B5, EIF2BE
Sequence domains: eIF4-gamma/eIF5/eIF2-epsilon
Translation initiation factor eIF2B subunit beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 368 amino acids
Theoretical weight: 41.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49770 (Residues: 2-351; Coverage: 100%)
Gene names: EIF2B2, EIF2BB
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit delta Chains: E, F
Molecule details ›
Chains: E, F
Length: 523 amino acids
Theoretical weight: 57.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UI10 (Residues: 1-523; Coverage: 100%)
Gene names: EIF2B4, EIF2BD
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit alpha Chains: G, H
Molecule details ›
Chains: G, H
Length: 305 amino acids
Theoretical weight: 33.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14232 (Residues: 1-305; Coverage: 100%)
Gene names: EIF2B1, EIF2BA
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit gamma Chains: I, J
Molecule details ›
Chains: I, J
Length: 452 amino acids
Theoretical weight: 50.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NR50 (Residues: 1-452; Coverage: 100%)
Gene name: EIF2B3
Sequence domains: Nucleotidyl transferase
Eukaryotic translation initiation factor 2 subunit 1 Chains: L, M
Molecule details ›
Chains: L, M
Length: 315 amino acids
Theoretical weight: 36.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05198 (Residues: 1-315; Coverage: 100%)
Gene names: EIF2A, EIF2S1
Sequence domains:
Eukaryotic translation initiation factor 2 subunit 3 Chains: S, T
Molecule details ›
Chains: S, T
Length: 472 amino acids
Theoretical weight: 51.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P41091 (Residues: 1-472; Coverage: 100%)
Gene names: EIF2G, EIF2S3
Sequence domains:
Translation initiation factor eiF2 beta-subunit Chains: d, e
Molecule details ›
Chains: d, e
Length: 14 amino acids
Theoretical weight: 1.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand C7B 1 x C7B
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.21Å
Relevant EMDB volumes: EMD-0649
Expression system: Escherichia coli

Quick links

Citations

19 review citations

The integrated stress response: From mechanism to disease.
Costa-Mattioli et al. (2020)
18 more

4 mentions without citation

Regulation and function of elF2B in neurological and metabolic disorders.
Hanson et al. (2022)
3 more