6o8q

X-ray diffraction
3.22Å resolution

HUaa 19bp SYM DNA pH 4.5

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-120372 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding protein HU-alpha Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 91 amino acids
Theoretical weight: 9.61 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0ACF0 (Residues: 1-90; Coverage: 100%)
Gene names: JW3964, b4000, hupA
Sequence domains: Bacterial DNA-binding protein
DNA (57-MER) Chain: K
Molecule details ›
Chain: K
Length: 57 nucleotides
Theoretical weight: 17.48 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (57-MER) Chain: L
Molecule details ›
Chain: L
Length: 57 nucleotides
Theoretical weight: 17.32 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 59.411Å b: 61.151Å c: 351.203Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.247 0.285
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided