› 6of8

X-ray diffraction
2.1Å resolution

Structure of Thr354Asn, Glu355Gln, Thr412Asn, Ile414Met, Ile464His, and Phe467Met mutant human CamKII-alpha hub domain

Released:
DOI: 10.2210/pdb6of8/pdb
PDB entry 6of8 coloured by chain, front view.
PDB entry 6of8 coloured by chain, side view.
PDB entry 6of8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Variation in assembly stoichiometry in non-metazoan homologs of the hub domain of Ca2+ /calmodulin-dependent protein kinase II.
McSpadden ED, Xia Z, Chi CC, Susa AC, Shah NH, Gee CL, Williams ER, Kuriyan J
Protein Sci 28 1071-1082 (2019)
PMID: 30942928

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194072 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium/calmodulin-dependent protein kinase type II subunit alpha Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 135 amino acids
Theoretical weight: 15.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UQM7 (Residues: 345-475; Coverage: 27%)
Gene names: CAMK2A, CAMKA, KIAA0968
Sequence domains: Calcium/calmodulin dependent protein kinase II association domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

2 bound ligands:
Ligand K 5 x K
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 162.985Å b: 121.343Å c: 56.239Å
α: 90° β: 108.15° γ: 90°
R-values:
R R work R free
0.224 0.222 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Structural Insights into the Regulation of Ca2+/Calmodulin-Dependent Protein Kinase II (CaMKII).
Bhattacharyya et al. (2020)
6 other articles

1 mention without citation

Variation in assembly stoichiometry in non-metazoan homologs of the hub domain of Ca2+ /calmodulin-dependent protein kinase II.
McSpadden et al. (2019)