› 6oi7

X-ray diffraction
2.9Å resolution

Se-Met structure of apo- Escherichia coli dGTPase

Released:
DOI: 10.2210/pdb6oi7/pdb
PDB entry 6oi7 coloured by chain, front view.
PDB entry 6oi7 coloured by chain, side view.
PDB entry 6oi7 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The crystal structure of dGTPase reveals the molecular basis of dGTP selectivity.
Barnes CO, Wu Y, Song J, Lin G, Baxter EL, Brewster AS, Nagarajan V, Holmes A, Soltis SM, Sauter NK, Ahn J, Cohen AE, Calero G
Proc Natl Acad Sci U S A 116 9333-9339 (2019)
PMID: 31019074

Function and Biology Details

Reaction catalysed: 
dGTP + H(2)O = deoxyguanosine + triphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147471 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyguanosinetriphosphate triphosphohydrolase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 505 amino acids
Theoretical weight: 59.99 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P15723 (Residues: 1-505; Coverage: 100%)
Gene names: JW0156, b0160, dgt
Sequence domains: HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

2 bound ligands:
Ligand MN 6 x MN
Ligand SO4 9 x SO4
1 modified residue:
Ligand MSE 66 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 192.183Å b: 192.183Å c: 287.189Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.234
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Bacterial origins of human cell-autonomous innate immune mechanisms.
Wein et al. (2022)
6 other articles

1 mention without citation

The crystal structure of dGTPase reveals the molecular basis of dGTP selectivity.
Barnes et al. (2019)