› 6okn

X-ray diffraction
3.25Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211246 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab 1A7 heavy chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 225 amino acids
Theoretical weight: 24.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Fab 1A7 light chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 214 amino acids
Theoretical weight: 23.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Tumor necrosis factor receptor superfamily member 4 Chains: E, R
Molecule details ›
Chains: E, R
Length: 163 amino acids
Theoretical weight: 17.7 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P43489 (Residues: 29-170; Coverage: 57%)
Gene names: TNFRSF4, TXGP1L
Sequence domains: TNFR/NGFR cysteine-rich region

Ligands and Environments

1 bound ligand:
Ligand EPE 3 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 121.59Å b: 121.59Å c: 198.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.228
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

8 review citations

Avidity in antibody effector functions and biotherapeutic drug design.
Oostindie et al. (2022)
7 more

1 mention without citation

Tetravalent biepitopic targeting enables intrinsic antibody agonism of tumor necrosis factor receptor superfamily members.
Yang et al. (2019)