› 6onp

X-ray diffraction
2.27Å resolution

Crystal structure of periplasmic binding protein XoxJ from Methylobacterium extorquens AM1

Released:
DOI: 10.2210/pdb6onp/pdb
PDB entry 6onp coloured by chain, front view.
PDB entry 6onp coloured by chain, side view.
PDB entry 6onp coloured by chain, top view.
Source organism: Methylorubrum extorquens AM1
Primary publication:
Biochemical and Structural Characterization of XoxG and XoxJ and Their Roles in Lanthanide-Dependent Methanol Dehydrogenase Activity.
Featherston ER, Rose HR, McBride MJ, Taylor EM, Boal AK, Cotruvo JA
Chembiochem 20 2360-2372 (2019)
PMID: 31017712

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111460 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Solute-binding protein family 3/N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 31.13 KDa
Source organism: Methylorubrum extorquens AM1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C5B122 (Residues: 1-284; Coverage: 100%)
Gene names: MexAM1_META1p1742, xoxJ
Sequence domains: Bacterial extracellular solute-binding proteins, family 3

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 49.9Å b: 51.759Å c: 51.241Å
α: 90° β: 98.237° γ: 90°
R-values:
R R work R free
0.241 0.24 0.279
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

The Chemistry of Lanthanides in Biology: Recent Discoveries, Emerging Principles, and Technological Applications.
Cotruvo JA. (2019)
8 other articles

1 mention without citation

Biochemical and Structural Characterization of XoxG and XoxJ and Their Roles in Lanthanide-Dependent Methanol Dehydrogenase Activity.
Featherston et al. (2019)