› 6osa

Electron Microscopy
3Å resolution

human Neurotensin Receptor 1 (hNTSR1) - Gi1 Protein Complex in non-canonical conformation (NC state)

Released:
DOI: 10.2210/pdb6osa/pdb
PDB entry 6osa coloured by chain, front view.
PDB entry 6osa coloured by chain, side view.
PDB entry 6osa coloured by chain, top view.
Source organisms:
Primary publication:
Conformational transitions of a neurotensin receptor 1-Gi1 complex.
Kato HE, Zhang Y, Hu H, Suomivuori CM, Kadji FMN, Aoki J, Krishna Kumar K, Fonseca R, Hilger D, Huang W, Latorraca NR, Inoue A, Dror RO, Kobilka BK, Skiniotis G
Nature 572 80-85 (2019)
PMID: 31243364
Related structures: EMD-20181

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-151865 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Neurotensin receptor type 1 Chain: R
Molecule details ›
Chain: R
Length: 435 amino acids
Theoretical weight: 48.4 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P30989 (Residues: 20-418; Coverage: 93%)
Gene names: NTRR, NTSR1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
JMV449 Chain: L
Molecule details ›
Chain: L
Length: 6 amino acids
Theoretical weight: 763 Da
Source organism: synthetic construct
Expression system: Not provided
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 344 amino acids
Theoretical weight: 37.67 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-20181
Expression systems:
  • Spodoptera frugiperda
  • Not provided
  • Trichoplusia ni

Quick links

Citations

17 review citations

G protein-coupled receptor-effector macromolecular membrane assemblies (GEMMAs).
Ferré et al. (2022)
16 more

16 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
15 more