› 6ou3

X-ray diffraction
1.8Å resolution

Crystal Structure of the D478S Variant of the Myocilin Olfactomedin Domain

Released:
DOI: 10.2210/pdb6ou3/pdb
PDB entry 6ou3 coloured by chain, front view.
PDB entry 6ou3 coloured by chain, side view.
PDB entry 6ou3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Stable calcium-free myocilin olfactomedin domain variants reveal challenges in differentiating between benign and glaucoma-causing mutations.
Hill SE, Kwon MS, Martin MD, Suntharalingam A, Hazel A, Dickey CA, Gumbart JC, Lieberman RL
J Biol Chem 294 12717-12728 (2019)
PMID: 31270212

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myocilin, C-terminal fragment Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 31.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99972 (Residues: 228-504; Coverage: 59%)
Gene names: GLC1A, MYOC, TIGR
Sequence domains: Olfactomedin-like domain

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 45.353Å b: 57.241Å c: 90.474Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.15 0.173
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Molecular Insights into Myocilin and Its Glaucoma-Causing Misfolded Olfactomedin Domain Variants.
Lieberman et al. (2021)
2 more

3 mentions without citation

Stable calcium-free myocilin olfactomedin domain variants reveal challenges in differentiating between benign and glaucoma-causing mutations.
Hill et al. (2019)
2 more