› 6p1a

X-ray diffraction
2.84Å resolution

Transcription antitermination factor Q21 in complex with Q21-binding-element DNA

Released:
DOI: 10.2210/pdb6p1a/pdb
PDB entry 6p1a coloured by chain, front view.
PDB entry 6p1a coloured by chain, side view.
PDB entry 6p1a coloured by chain, top view.
Source organism: Phage 21
Primary publication:
Structural basis of Q-dependent antitermination.
Yin Z, Kaelber JT, Ebright RH
Proc Natl Acad Sci U S A 116 18384-18390 (2019)
PMID: 31455742

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-120420 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Q protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 162 amino acids
Theoretical weight: 18.67 KDa
Source organism: Phage 21
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9XJQ6 (Residues: 2-23, 24-161; Coverage: 99%)
Gene name: Q
Sequence domains: Phage antitermination protein Q
DNA (5'-D(P*CP*TP*CP*AP*TP*TP*GP*AP*GP*CP*AP*AP*AP*TP*GP*AP*GP*CP*AP*AP*G)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 21 nucleotides
Theoretical weight: 6.48 KDa
Source organism: Phage 21
Expression system: Not provided
DNA (5'-D(*CP*TP*TP*GP*CP*TP*CP*AP*TP*TP*TP*GP*CP*TP*CP*AP*AP*TP*GP*AP*G)-3') Chains: E, G
Molecule details ›
Chains: E, G
Length: 21 nucleotides
Theoretical weight: 6.4 KDa
Source organism: Phage 21
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand EDO 3 x EDO
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P21212
Unit cell:
a: 69.573Å b: 263.224Å c: 56.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.231 0.275
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

4 review citations

Coupled Transcription-Translation in Prokaryotes: An Old Couple With New Surprises.
Irastortza-Olaziregi et al. (2020)
3 more

1 mention without citation

Structural basis of Q-dependent antitermination.
Yin et al. (2019)