› 6pmo

X-ray diffraction
2.66Å resolution

Co-crystal structure of the Geobacillus kaustophilus glyQ T-box riboswitch discriminator domain in complex with tRNA-Gly

Released:
DOI: 10.2210/pdb6pmo/pdb
PDB entry 6pmo coloured by chain, front view.
PDB entry 6pmo coloured by chain, side view.
PDB entry 6pmo coloured by chain, top view.
Source organism: Geobacillus kaustophilus
Primary publication:
Structural basis of amino acid surveillance by higher-order tRNA-mRNA interactions.
Li S, Su Z, Lehmann J, Stamatopoulou V, Giarimoglou N, Henderson FE, Fan L, Pintilie GD, Zhang K, Chen M, Ludtke SJ, Wang YX, Stathopoulos C, Chiu W, Zhang J
Nat Struct Mol Biol 26 1094-1105 (2019)
PMID: 31740854

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195827 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
tRNA-Gly Chain: B
Molecule details ›
Chain: B
Length: 75 nucleotides
Theoretical weight: 24.16 KDa
Source organism: Geobacillus kaustophilus
Expression system: Not provided
Sequence domains: tRNA
T-box riboswitch discriminator Chain: A
Molecule details ›
Chain: A
Length: 66 nucleotides
Theoretical weight: 21.37 KDa
Source organism: Geobacillus kaustophilus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand IR 13 x IR
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 44.851Å b: 139.913Å c: 136.023Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.225 0.273
Expression system: Not provided

Quick links

Citations

13 review citations

Cryo-EM advances in RNA structure determination.
Ma et al. (2022)
12 more

7 mentions without citation

Unboxing the T-box riboswitches-A glimpse into multivalent and multimodal RNA-RNA interactions.
Zhang J. (2020)
6 more