› 6poo

X-ray diffraction
3.03Å resolution

Novel structure of the N-terminal helical domain of BibA, a group B streptococcus immunogenic bacterial adhesin

Released:
DOI: 10.2210/pdb6poo/pdb
PDB entry 6poo coloured by chain, front view.
PDB entry 6poo coloured by chain, side view.
PDB entry 6poo coloured by chain, top view.
Source organism: Streptococcus agalactiae
Primary publication:
Novel structure of the N-terminal helical domain of BibA, a group B streptococcus immunogenic bacterial adhesin.
Manne K, Chattopadhyay D, Agarwal V, Blom AM, Khare B, Chakravarthy S, Chang C, Ton-That H, Narayana SVL
Acta Crystallogr D Struct Biol 76 759-770 (2020)
PMID: 32744258

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184264 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gram-positive cocci surface proteins LPxTG domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 273 amino acids
Theoretical weight: 30.97 KDa
Source organism: Streptococcus agalactiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8DWZ6 (Residues: 126-398; Coverage: 46%)
Gene name: SAG2063
Sequence domains: BibA adhesin stalk domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Unit cell:
a: 26.419Å b: 68.814Å c: 199.377Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.24
Expression system: Escherichia coli

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Citations

1 review citation

Immunogenic Proteins of Group B Streptococcus-Potential Antigens in Immunodiagnostic Assay for GBS Detection.
Dobrut et al. (2021)
2 other articles

4 mentions without citation

Implications of AlphaFold2 for crystallographic phasing by molecular replacement.
McCoy et al. (2022)
3 more