Function and Biology Details
Reaction catalysed:
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132771 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperone protein DnaJ 2
Molecule details ›
Chains: A, C
Length: 280 amino acids
Theoretical weight: 31.02 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 280 amino acids
Theoretical weight: 31.02 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q56237 (Residues: 1-280; Coverage: 100%)
Sequence domains:
Alkaline phosphatase
Molecule details ›
Chain: B
Length: 471 amino acids
Theoretical weight: 49.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Alkaline phosphatase
Length: 471 amino acids
Theoretical weight: 49.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P00634 (Residues: 1-471; Coverage: 100%)
Sequence domains: Alkaline phosphatase
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Chemical shift assignment:
22%
Refinement method:
molecular dynamics
Expression system: Escherichia coli BL21(DE3)
