› 6pv8

Electron Microscopy
3.87Å resolution

Human alpha3beta4 nicotinic acetylcholine receptor in complex with AT-1001

Released:
DOI: 10.2210/pdb6pv8/pdb
PDB entry 6pv8 coloured by chain, front view.
PDB entry 6pv8 coloured by chain, side view.
PDB entry 6pv8 coloured by chain, top view.
Source organisms:
Primary publication:
Agonist Selectivity and Ion Permeation in the α3β4 Ganglionic Nicotinic Receptor.
Gharpure A, Teng J, Zhuang Y, Noviello CM, Walsh RM, Cabuco R, Howard RJ, Zaveri NT, Lindahl E, Hibbs RE
Neuron 104 501-511.e6 (2019)
PMID: 31488329
Related structures: EMD-20488

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-211023 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (7 distinct):
Neuronal acetylcholine receptor subunit alpha-3 Chains: A, D
Molecule details ›
Chains: A, D
Length: 525 amino acids
Theoretical weight: 60.09 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P32297 (Residues: 32-378, 379-386, 387-398, 399-431, 434-505; Coverage: 100%)
Gene names: CHRNA3, NACHRA3
Sequence domains:
Neuronal acetylcholine receptor subunit beta-4 Chains: B, C, E
Molecule details ›
Chains: B, C, E
Length: 538 amino acids
Theoretical weight: 61.34 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P30926 (Residues: 22-363, 364-374, 375-379, 380-398, 399-416, 417-498; Coverage: 100%)
Gene name: CHRNB4
Sequence domains:
IgG2b Fab heavy chain Chains: F, H
Molecule details ›
Chains: F, H
Length: 219 amino acids
Theoretical weight: 23.4 KDa
Source organism: Mus musculus
Kappa Fab light chain Chains: G, I
Molecule details ›
Chains: G, I
Length: 213 amino acids
Theoretical weight: 23.4 KDa
Source organism: Mus musculus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
4 bound ligands:
Ligand P1M 2 x P1M
Ligand Y01 10 x Y01
Ligand NAG 6 x NAG
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.87Å
Relevant EMDB volumes: EMD-20488
Expression system: Homo sapiens

Quick links

Citations

17 review citations

Nicotinic acetylcholine receptors: Conventional and unconventional ligands and signaling.
Papke et al. (2020)
16 more

6 mentions without citation

Pursuing High-Resolution Structures of Nicotinic Acetylcholine Receptors: Lessons Learned from Five Decades.
Delgado-Vélez et al. (2021)
5 more