› 6pw9

Electron Microscopy
4.03Å resolution

Cryo-EM structure of human NatE/HYPK complex

Released:
DOI: 10.2210/pdb6pw9/pdb
PDB entry 6pw9 coloured by chain, front view.
PDB entry 6pw9 coloured by chain, side view.
PDB entry 6pw9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for N-terminal acetylation by human NatE and its modulation by HYPK.
Deng S, McTiernan N, Wei X, Arnesen T, Marmorstein R
Nat Commun 11 818 (2020)
PMID: 32042062
Related structures: EMD-20501

Function and Biology Details

Reactions catalysed: 
Acetyl-CoA + an N-terminal-L-methionyl-L-alanyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-alanyl-[protein] + CoA
Acetyl-CoA + an N-terminal-L-alanyl-[protein] = an N-terminal-N(alpha)-acetyl-L-alanyl-[protein] + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
N-alpha-acetyltransferase 50 Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GZZ1 (Residues: 1-169; Coverage: 100%)
Gene names: MAK3, NAA50, NAT13, NAT5
Sequence domains: Acetyltransferase (GNAT) family
N-alpha-acetyltransferase 15, NatA auxiliary subunit Chain: B
Molecule details ›
Chain: B
Length: 866 amino acids
Theoretical weight: 101.43 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BXJ9 (Residues: 1-866; Coverage: 100%)
Gene names: GA19, NAA15, NARG1, NATH, TBDN100
Sequence domains:
N-alpha-acetyltransferase 10 Chain: C
Molecule details ›
Chain: C
Length: 236 amino acids
Theoretical weight: 26.52 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41227 (Residues: 1-235; Coverage: 100%)
Gene names: ARD1, ARD1A, NAA10, TE2
Sequence domains: Acetyltransferase (GNAT) family
Huntingtin-interacting protein K Chain: D
Molecule details ›
Chain: D
Length: 129 amino acids
Theoretical weight: 14.69 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NX55 (Residues: 1-121; Coverage: 100%)
Gene names: C15orf63, HSPC136, HYPK
Sequence domains: HYPK UBA domain

Ligands and Environments


Cofactor: Ligand ACO 1 x ACO
1 bound ligand:
Ligand IHP 1 x IHP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.03Å
Relevant EMDB volumes: EMD-20501
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

5 review citations

How Elongator Acetylates tRNA Bases.
Abbassi et al. (2020)
4 more

2 mentions without citation

Protein N-Terminal Acetylation: Structural Basis, Mechanism, Versatility, and Regulation.
Deng et al. (2021)
1 more