› 6q14

Electron Microscopy
3.8Å resolution

Structure of the Salmonella SPI-1 injectisome NC-base

Released:
DOI: 10.2210/pdb6q14/pdb
PDB entry 6q14 coloured by chain, front view.
PDB entry 6q14 coloured by chain, side view.
PDB entry 6q14 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
T3S injectisome needle complex structures in four distinct states reveal the basis of membrane coupling and assembly.
Hu J, Worrall LJ, Vuckovic M, Hong C, Deng W, Atkinson CE, Brett Finlay B, Yu Z, Strynadka NCJ
Nat Microbiol 4 2010-2019 (2019)
PMID: 31427728
Related structures: EMD-20310

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 74-mer (preferred)
PDBe Complex ID:
PDB-CPX-141021 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Lipoprotein PrgK Chains: AA, AB, AC, AD, AE, AF, AG, AH, AI, AJ, AK, AL, o, p, q, r, s, t, u, v, w, x, y, z
Molecule details ›
Chains: AA, AB, AC, AD, AE, AF, AG, AH, AI, AJ, AK, AL, o, p, q, r, s, t, u, v, w, x, y, z
Length: 252 amino acids
Theoretical weight: 28.25 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P41786 (Residues: 1-252; Coverage: 100%)
Gene names: STM2871, prgK
Sequence domains: Secretory protein of YscJ/FliF family
Surface presentation of antigens protein SpaP Chains: 0, 1, 2, 3, 4
Molecule details ›
Chains: 0, 1, 2, 3, 4
Length: 224 amino acids
Theoretical weight: 25.25 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P40700 (Residues: 1-224; Coverage: 100%)
Gene names: STM2890, spaP
Sequence domains: FliP family
Surface presentation of antigens protein SpaR Chain: 5
Molecule details ›
Chain: 5
Length: 263 amino acids
Theoretical weight: 28.5 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P40701 (Residues: 1-263; Coverage: 100%)
Gene names: STM2888, spaR
Sequence domains: Bacterial export proteins, family 1
Surface presentation of antigens protein SpaQ Chains: 6, 7, 8, 9
Molecule details ›
Chains: 6, 7, 8, 9
Length: 86 amino acids
Theoretical weight: 9.36 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1L7 (Residues: 1-86; Coverage: 100%)
Gene names: STM2889, spaQ
Sequence domains: Bacterial export proteins, family 3
Protein PrgH Chains: E, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j, k, l, m, n
Molecule details ›
Chains: E, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j, k, l, m, n
Length: 392 amino acids
Theoretical weight: 44.51 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P41783 (Residues: 1-392; Coverage: 100%)
Gene names: STM2874, prgH
Sequence domains: Type III secretion system protein PrgH-EprH (PrgH)
SPI-1 type 3 secretion system secretin Chains: A, B, C, D, F, G, H, I, J, K, L, M, N, O, P, Q
Molecule details ›
Chains: A, B, C, D, F, G, H, I, J, K, L, M, N, O, P, Q
Length: 562 amino acids
Theoretical weight: 61.84 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P35672 (Residues: 1-562; Coverage: 100%)
Gene names: STM2898, invG, sctC1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-20310

Quick links

Citations

7 review citations

Molecular Targets and Strategies for Inhibition of the Bacterial Type III Secretion System (T3SS); Inhibitors Directly Binding to T3SS Components.
Hotinger et al. (2021)
6 more

83 mentions without citation

The role of neuronal complexes in human X-linked brain diseases.
Laumonnier et al. (2007)
82 more