› 6q18

X-ray diffraction
2.55Å resolution

Human antibody H1244 in complex with the influenza hemagglutinin head domain of A/Beijing/262/95(H1N1)

Released:
DOI: 10.2210/pdb6q18/pdb
PDB entry 6q18 coloured by chain, front view.
PDB entry 6q18 coloured by chain, side view.
PDB entry 6q18 coloured by chain, top view.
Source organisms:
Primary publication:
Affinity maturation in a human humoral response to influenza hemagglutinin.
McCarthy KR, Raymond DD, Do KT, Schmidt AG, Harrison SC
Proc Natl Acad Sci U S A (2019)
PMID: 31843892

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207673 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Hemagglutinin HA1 chain Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 25.34 KDa
Source organism: Influenza A virus (A/Beijing/262/1995(H1N1))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: B4UPF7 (Residues: 65-280; Coverage: 38%)
Gene name: HA
Sequence domains: Haemagglutinin
H1244 Fab lambda chain Chain: L
Molecule details ›
Chain: L
Length: 217 amino acids
Theoretical weight: 23.07 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
H1244 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 243 amino acids
Theoretical weight: 25.87 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

2 bound ligands:
Ligand NAG 2 x NAG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 58Å b: 69.64Å c: 191.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.243
Expression systems:
  • Trichoplusia ni
  • Homo sapiens

Quick links

Citations

4 review citations

B Cell Responses against Influenza Viruses: Short-Lived Humoral Immunity against a Life-Long Threat.
Guthmiller et al. (2021)
3 more

2 mentions without citation

Affinity maturation in a human humoral response to influenza hemagglutinin.
McCarthy et al. (2019)
1 more