› 6q2j

Electron Microscopy
4.1Å resolution

Cryo-EM structure of extracellular dimeric complex of RET/GFRAL/GDF15

Released:
DOI: 10.2210/pdb6q2j/pdb
PDB entry 6q2j coloured by chain, front view.
PDB entry 6q2j coloured by chain, side view.
PDB entry 6q2j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM analyses reveal the common mechanism and diversification in the activation of RET by different ligands.
Li J, Shang G, Chen YJ, Brautigam CA, Liou J, Zhang X, Bai XC
Elife 8 (2019)
PMID: 31535977
Related structures: EMD-20572

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-139857 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Growth/differentiation factor 15 Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 14.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99988 (Residues: 197-308; Coverage: 40%)
Gene names: GDF15, MIC1, PDF, PLAB, PTGFB
Sequence domains: Transforming growth factor beta like domain
GDNF family receptor alpha-like Chains: C, D
Molecule details ›
Chains: C, D
Length: 343 amino acids
Theoretical weight: 39.12 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6UXV0 (Residues: 20-352; Coverage: 89%)
Gene names: C6orf144, GFRAL, UNQ9356/PRO34128
Sequence domains: GDNF/GAS1 domain
Extracellular cell-membrane anchored RET cadherin 120 kDa fragment Chains: E, F
Molecule details ›
Chains: E, F
Length: 617 amino acids
Theoretical weight: 69.1 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P07949 (Residues: 29-635; Coverage: 56%)
Gene names: CDHF12, CDHR16, PTC, RET
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand NAG 14 x NAG
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-20572
Expression systems:
  • Escherichia coli
  • Homo sapiens

Quick links

Citations

7 review citations

GDF15: emerging biology and therapeutic applications for obesity and cardiometabolic disease.
Wang et al. (2021)
6 more

7 mentions without citation

Receptor tyrosine kinase activation: From the ligand perspective.
Trenker et al. (2020)
6 more