6q2r

Electron Microscopy
4.3Å resolution

Cryo-EM structure of RET/GFRa2/NRTN extracellular complex in the tetrameric form

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-126292 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Neurturin Chains: A, B, U, V
Molecule details ›
Chains: A, B, U, V
Length: 102 amino acids
Theoretical weight: 11.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99748 (Residues: 96-197; Coverage: 57%)
Gene name: NRTN
Sequence domains: Transforming growth factor beta like domain
GDNF family receptor alpha-2 Chains: C, D, W, X
Molecule details ›
Chains: C, D, W, X
Length: 349 amino acids
Theoretical weight: 39.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O00451 (Residues: 24-362; Coverage: 77%)
Gene names: GDNFRB, GFRA2, RETL2, TRNR2
Sequence domains: GDNF/GAS1 domain
Extracellular cell-membrane anchored RET cadherin 120 kDa fragment Chains: E, F, Y, Z
Molecule details ›
Chains: E, F, Y, Z
Length: 617 amino acids
Theoretical weight: 69.1 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P07949 (Residues: 29-635; Coverage: 56%)
Gene names: CDHF12, CDHR16, PTC, RET
Sequence domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-20578
Expression systems:
  • Escherichia coli
  • Homo sapiens