› 6q2s

Electron Microscopy
3.8Å resolution

Cryo-EM structure of RET/GFRa3/ARTN extracellular complex. The 3D refinement was applied with C2 symmetry.

Released:
DOI: 10.2210/pdb6q2s/pdb
PDB entry 6q2s coloured by chain, front view.
PDB entry 6q2s coloured by chain, side view.
PDB entry 6q2s coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM analyses reveal the common mechanism and diversification in the activation of RET by different ligands.
Li J, Shang G, Chen YJ, Brautigam CA, Liou J, Zhang X, Bai XC
Elife 8 (2019)
PMID: 31535977
Related structures: EMD-20579

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-130047 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Artemin; Ubiquitin-like protein SMT3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 235 amino acids
Theoretical weight: 25.79 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q12306 (Residues: 1-98; Coverage: 97%)
  • Canonical: Q5T4W7 (Residues: 108-220; Coverage: 62%)
Gene names: ARTN, D9719.15, EVN, SMT3, YDR510W
Sequence domains:
GDNF family receptor alpha-3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 342 amino acids
Theoretical weight: 38.37 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O60609 (Residues: 32-363; Coverage: 90%)
Gene names: GFRA3, UNQ339/PRO538/PRO3664
Sequence domains: GDNF/GAS1 domain
Extracellular cell-membrane anchored RET cadherin 120 kDa fragment Chains: E, F
Molecule details ›
Chains: E, F
Length: 617 amino acids
Theoretical weight: 69.1 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P07949 (Residues: 29-635; Coverage: 56%)
Gene names: CDHF12, CDHR16, PTC, RET
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand NAG 14 x NAG
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-20579
Expression systems:
  • Escherichia coli
  • Homo sapiens

Quick links

Citations

7 review citations

GDF15: emerging biology and therapeutic applications for obesity and cardiometabolic disease.
Wang et al. (2021)
6 more

4 mentions without citation

Receptor tyrosine kinase activation: From the ligand perspective.
Trenker et al. (2020)
3 more