› 6q9e

Electron Microscopy
3.9Å resolution

Complex III2 focused refinement from Ovine respiratory supercomplex I+III2

Released:
DOI: 10.2210/pdb6q9e/pdb
PDB entry 6q9e coloured by chain, front view.
PDB entry 6q9e coloured by chain, side view.
PDB entry 6q9e coloured by chain, top view.
Source organism: Ovis aries
Primary publication:
Structures of Respiratory Supercomplex I+III2 Reveal Functional and Conformational Crosstalk.
Letts JA, Fiedorczuk K, Degliesposti G, Skehel M, Sazanov LA
Mol Cell 75 1131-1146.e6 (2019)
PMID: 31492636
Related structures: EMD-4481

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
PDBe Complex ID:
PDB-CPX-150326 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
Cytochrome b-c1 complex subunit 1, mitochondrial Chains: a1, a3
Molecule details ›
Chains: a1, a3
Length: 446 amino acids
Theoretical weight: 49.39 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5Q5G6 (Residues: 35-483; Coverage: 92%)
Gene name: LOC101110095
Sequence domains:
Ubiquinol-cytochrome c reductase core protein 2 Chains: a2, a4
Molecule details ›
Chains: a2, a4
Length: 439 amino acids
Theoretical weight: 46.66 KDa
Source organism: Ovis aries
Cytochrome b Chains: b1, b2
Molecule details ›
Chains: b1, b2
Length: 379 amino acids
Theoretical weight: 42.88 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: P24959 (Residues: 1-379; Coverage: 100%)
Gene names: COB, CYTB, MT-CYB, MTCYB
Sequence domains:
Cytochrome c1 Chains: c1, c2
Molecule details ›
Chains: c1, c2
Length: 240 amino acids
Theoretical weight: 27.32 KDa
Source organism: Ovis aries
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chains: f1, f2
Molecule details ›
Chains: f1, f2
Length: 196 amino acids
Theoretical weight: 21.65 KDa
Source organism: Ovis aries
Cytochrome b-c1 complex subunit 7 Chains: d1, d2
Molecule details ›
Chains: d1, d2
Length: 110 amino acids
Theoretical weight: 13.43 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5P642 (Residues: 1-109; Coverage: 100%)
Sequence domains: Ubiquinol-cytochrome C reductase complex 14kD subunit
Cytochrome b-c1 complex subunit 8 Chains: q1, q2
Molecule details ›
Chains: q1, q2
Length: 81 amino acids
Theoretical weight: 9.61 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PUP9 (Residues: 2-82; Coverage: 99%)
Gene name: LOC101121420
Sequence domains: UcrQ family
Cytochrome b-c1 complex subunit 6 Chains: h1, h2
Molecule details ›
Chains: h1, h2
Length: 78 amino acids
Theoretical weight: 9.22 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PZC9 (Residues: 14-91; Coverage: 86%)
Gene name: LOC101115589
Sequence domains: Ubiquinol-cytochrome C reductase hinge protein
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chains: x1, x2
Molecule details ›
Chains: x1, x2
Length: 33 amino acids
Theoretical weight: 2.83 KDa
Source organism: Ovis aries
Ubiquinol-cytochrome c reductase, complex III subunit X Chains: i1, i2
Molecule details ›
Chains: i1, i2
Length: 63 amino acids
Theoretical weight: 7.31 KDa
Source organism: Ovis aries

Ligands and Environments

6 bound ligands:
Ligand HEM 4 x HEM
Ligand 3PE 4 x 3PE
Ligand CDL 5 x CDL
Ligand U10 3 x U10
Ligand HEC 2 x HEC
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-4481

Quick links

Citations

19 review citations

Generation of Reactive Oxygen Species by Mitochondria.
Hernansanz-Agustín et al. (2021)
18 more

10 mentions without citation

Mitochondrial Metabolism as a Target for Cancer Therapy.
Vasan et al. (2020)
9 more