6qhd

X-ray diffraction
2.85Å resolution

Lysine acetylated and tyrosine phosphorylated STAT3 in a complex with DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-120489 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Signal transducer and activator of transcription 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 596 amino acids
Theoretical weight: 68.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P40763 (Residues: 127-715; Coverage: 77%)
Gene names: APRF, STAT3
Sequence domains:
DNA (5'-D(*AP*AP*GP*AP*TP*TP*TP*AP*CP*GP*GP*GP*AP*AP*AP*TP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 18 nucleotides
Theoretical weight: 5.59 KDa
Source organism: Murine adenovirus 1
Expression system: Not provided
DNA (5'-D(*TP*GP*CP*AP*TP*TP*TP*CP*CP*CP*GP*TP*AP*AP*AP*TP*CP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.44 KDa
Source organism: Murine adenovirus 1
Expression system: Not provided

Ligands and Environments

No bound ligands
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P41
Unit cell:
a: 175.491Å b: 175.491Å c: 79.078Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.297 0.294 0.343
Expression systems:
  • Escherichia coli
  • Not provided