› 6qxf

Electron Microscopy
3.6Å resolution

Cas1-Cas2-Csn2-DNA complex from the Type II-A CRISPR-Cas system

Released:
DOI: 10.2210/pdb6qxf/pdb
PDB entry 6qxf coloured by chain, front view.
PDB entry 6qxf coloured by chain, side view.
PDB entry 6qxf coloured by chain, top view.
Source organism: Streptococcus thermophilus
Primary publication:
Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System.
Wilkinson M, Drabavicius G, Silanskas A, Gasiunas G, Siksnys V, Wigley DB
Mol Cell 75 90-101.e5 (2019)
PMID: 31080012
Related structures: EMD-4668

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 22-mer (preferred)
PDBe Complex ID:
PDB-CPX-120514 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
CRISPR-associated protein Csn2 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 219 amino acids
Theoretical weight: 25.53 KDa
Source organism: Streptococcus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3ECR4 (Residues: 1-219; Coverage: 100%)
Gene name: csn2
Sequence domains: CRISPR-associated protein (Cas_Csn2)
Structure domains:
CRISPR-associated endonuclease Cas1 Chains: I, J, L, M, O, P, R, S
Molecule details ›
Chains: I, J, L, M, O, P, R, S
Length: 302 amino acids
Theoretical weight: 35.36 KDa
Source organism: Streptococcus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3ECR2 (Residues: 1-289; Coverage: 100%)
Gene name: cas1
Sequence domains: CRISPR associated protein Cas1
CRISPR-associated endoribonuclease Cas2 Chains: K, N, Q, T
Molecule details ›
Chains: K, N, Q, T
Length: 114 amino acids
Theoretical weight: 13.43 KDa
Source organism: Streptococcus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3ECR3 (Residues: 1-114; Coverage: 100%)
Gene name: cas2
Sequence domains: CRISPR associated protein Cas2
DNA (25-MER) Chain: X
Molecule details ›
Chain: X
Length: 25 nucleotides
Theoretical weight: 7.56 KDa
DNA (25-MER) Chain: Y
Molecule details ›
Chain: Y
Length: 25 nucleotides
Theoretical weight: 7.79 KDa

Ligands and Environments

1 bound ligand:
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-4668
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

CRISPR/Cas9 based genome editing for targeted transcriptional control in triple-negative breast cancer.
Deepak Singh et al. (2021)
8 more

1 mention without citation

Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System.
Wilkinson et al. (2019)