› 6r4e

X-ray diffraction
2.35Å resolution

Crystal structure of human GFAT-1 in complex with Glucose-6-Phosphate and L-Glu

Released:
DOI: 10.2210/pdb6r4e/pdb
PDB entry 6r4e coloured by chain, front view.
PDB entry 6r4e coloured by chain, side view.
PDB entry 6r4e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Loss of GFAT-1 feedback regulation activates the hexosamine pathway that modulates protein homeostasis.
Ruegenberg S, Horn M, Pichlo C, Allmeroth K, Baumann U, Denzel MS
Nat Commun 11 687 (2020)
PMID: 32019926

Function and Biology Details

Reaction catalysed: 
L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170307 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 687 amino acids
Theoretical weight: 77.78 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q06210 (Residues: 1-317, 318-699; Coverage: 97%)
Gene names: GFAT, GFPT, GFPT1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand G6Q 2 x G6Q
Ligand GLU 1 x GLU
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P41212
Unit cell:
a: 153.847Å b: 153.847Å c: 166.292Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.21
Expression system: Spodoptera frugiperda

Quick links

Citations

6 review citations

Integration of O-GlcNAc into Stress Response Pathways.
Fahie et al. (2022)
5 more

4 mentions without citation

Mechanisms of coordinating hyaluronan and glycosaminoglycan production by nucleotide sugars.
Zimmer et al. (2022)
3 more