6r8f

Electron Microscopy
3.8Å resolution

Cryo-EM structure of the Human BRISC-SHMT2 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-152741 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Lys-63-specific deubiquitinase BRCC36 Chains: A, C
Molecule details ›
Chains: A, C
Length: 316 amino acids
Theoretical weight: 36.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P46736 (Residues: 1-316; Coverage: 100%)
Gene names: BRCC3, BRCC36, C6.1A, CXorf53
Sequence domains:
BRISC complex subunit Abraxas 2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 267 amino acids
Theoretical weight: 31.03 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q15018 (Residues: 1-267; Coverage: 64%)
Gene names: ABRAXAS2, ABRO1, FAM175B, KIAA0157
Sequence domains: BRCA1-A complex subunit Abraxas 1 MPN domain
Serine hydroxymethyltransferase, mitochondrial Chains: K, L
Molecule details ›
Chains: K, L
Length: 504 amino acids
Theoretical weight: 56.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P34897 (Residues: 1-504; Coverage: 100%)
Gene name: SHMT2
Sequence domains: Serine hydroxymethyltransferase
BRISC and BRCA1-A complex member 2 Chains: E, G
Molecule details ›
Chains: E, G
Length: 217 amino acids
Theoretical weight: 22.03 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NXR7 (Residues: 1-133; Coverage: 35%)
Gene names: BABAM2, BRCC45, BRE
Sequence domains: Brain and reproductive organ-expressed protein (BRE)

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-4759
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli