6rjd

Electron Microscopy
3.3Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-120549 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9 1 Chain: C
Molecule details ›
Chain: C
Length: 1121 amino acids
Theoretical weight: 129.7 KDa
Source organism: Streptococcus thermophilus DGCC 7710
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03LF7 (Residues: 1-1121; Coverage: 100%)
Gene names: STER_0709, cas9-1, csn1
Sequence domains:
tDNA59 Chain: E
Molecule details ›
Chain: E
Length: 59 nucleotides
Theoretical weight: 18.16 KDa
Source organism: Streptococcus phage D1811
Expression system: Not provided
ntPAM Chain: G
Molecule details ›
Chain: G
Length: 23 nucleotides
Theoretical weight: 7.08 KDa
Source organism: Streptococcus virus 2972
Expression system: Not provided
sgRNA (78-MER) Chain: D
Molecule details ›
Chain: D
Length: 117 nucleotides
Theoretical weight: 37.44 KDa
Source organism: Streptococcus thermophilus DGCC 7710
Expression system: in vitro transcription vector pT7-Fluc(deltai)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-4902
Expression systems:
  • Escherichia coli
  • Not provided
  • in vitro transcription vector pT7-Fluc(deltai)