› 6rjg

Electron Microscopy
3.2Å resolution

Cryo-EM structure of St1Cas9-sgRNA-AcrIIA6-tDNA59-ntPAM complex.

Released:
DOI: 10.2210/pdb6rjg/pdb
PDB entry 6rjg coloured by chain, front view.
PDB entry 6rjg coloured by chain, side view.
PDB entry 6rjg coloured by chain, top view.
Source organisms:
Primary publication:
Cas9 Allosteric Inhibition by the Anti-CRISPR Protein AcrIIA6.
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell 76 922-937.e7 (2019)
PMID: 31604602
Related structures: EMD-4904

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104538 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Acr-like protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 21.46 KDa
Source organism: Streptococcus phage D1811
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2U7VKE8 (Residues: 1-183; Coverage: 100%)
Gene name: D1811_026
CRISPR-associated endonuclease Cas9 1 Chain: C
Molecule details ›
Chain: C
Length: 1121 amino acids
Theoretical weight: 129.7 KDa
Source organism: Streptococcus thermophilus DGCC 7710
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03LF7 (Residues: 1-1121; Coverage: 100%)
Gene names: STER_0709, cas9-1, csn1
Sequence domains:
tDNA59 Chain: E
Molecule details ›
Chain: E
Length: 59 nucleotides
Theoretical weight: 18.16 KDa
ntPAM Chain: G
Molecule details ›
Chain: G
Length: 23 nucleotides
Theoretical weight: 7.08 KDa
sgRNA Chain: D
Molecule details ›
Chain: D
Length: 117 nucleotides
Theoretical weight: 37.44 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-4904
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies.
Marino et al. (2020)
8 more

2 mentions without citation

Atomic-scale insights into allosteric inhibition and evolutional rescue mechanism of Streptococcus thermophilus Cas9 by the anti-CRISPR protein AcrIIA6.
Li et al. (2021)
1 more