› 6rrc

X-ray diffraction
2.37Å resolution

Crystal structure of the N-terminal region of human cohesin subunit STAG1 in complex with RAD21 peptide

Released:
DOI: 10.2210/pdb6rrc/pdb
PDB entry 6rrc coloured by chain, front view.
PDB entry 6rrc coloured by chain, side view.
PDB entry 6rrc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
STAG1 vulnerabilities for exploiting cohesin synthetic lethality in STAG2-deficient cancers.
van der Lelij P, Newman JA, Lieb S, Jude J, Katis V, Hoffmann T, Hinterndorfer M, Bader G, Kraut N, Pearson MA, Peters JM, Zuber J, Gileadi O, Petronczki M
Life Sci Alliance 3 (2020)
PMID: 32467316

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129913 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cohesin subunit SA-1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 339 amino acids
Theoretical weight: 39.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WVM7 (Residues: 86-420; Coverage: 27%)
Gene names: SA1, SCC3, STAG1
Sequence domains:
64-kDa C-terminal product Chains: B, D
Molecule details ›
Chains: B, D
Length: 25 amino acids
Theoretical weight: 2.9 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O60216 (Residues: 321-345; Coverage: 4%)
Gene names: HR21, KIAA0078, NXP1, RAD21, SCC1

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C222
Unit cell:
a: 117.077Å b: 151.662Å c: 126.656Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.214 0.248
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

Cohesin Mutations in Cancer: Emerging Therapeutic Targets.
Antony et al. (2021)
7 more