› 6rsy

X-ray diffraction
2.95Å resolution

The complex between TCR a7b2 and human Class I MHC HLA-A0201-WT1 with the bound RMFPNAPYL peptide.

Released:
DOI: 10.2210/pdb6rsy/pdb
PDB entry 6rsy coloured by chain, front view.
PDB entry 6rsy coloured by chain, side view.
PDB entry 6rsy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Specificity of bispecific T cell receptors and antibodies targeting peptide-HLA.
Holland CJ, Crean RM, Pentier JM, de Wet B, Lloyd A, Srikannathasan V, Lissin N, Lloyd KA, Blicher TH, Conroy PJ, Hock M, Pengelly RJ, Spinner TE, Cameron B, Potter EA, Jeyanthan A, Molloy PE, Sami M, Aleksic M, Liddy N, Robinson RA, Harper S, Lepore M, Pudney CR, van der Kamp MW, Rizkallah PJ, Jakobsen BK, Vuidepot A, Cole DK
J Clin Invest 130 2673-2688 (2020)
PMID: 32310221

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-209020 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, F
Molecule details ›
Chains: A, F
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Beta-2-microglobulin Chains: B, G
Molecule details ›
Chains: B, G
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Wilms tumor protein Chains: C, H
Molecule details ›
Chains: C, H
Length: 9 amino acids
Theoretical weight: 1.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19544 (Residues: 126-134; Coverage: 2%)
Gene name: WT1
a7b2 ALPHA CHAIN Chains: D, I
Molecule details ›
Chains: D, I
Length: 208 amino acids
Theoretical weight: 23.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
DMF4 Beta CHAIN Chains: E, J
Molecule details ›
Chains: E, J
Length: 245 amino acids
Theoretical weight: 28.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 94.61Å b: 114.75Å c: 185.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.251 0.291
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Immunoglobulins or Antibodies: IMGT® Bridging Genes, Structures and Functions.
Lefranc et al. (2020)
10 more

5 mentions without citation

TCR Recognition of Peptide-MHC-I: Rule Makers and Breakers.
Szeto et al. (2020)
4 more