› 6rtx

X-ray diffraction
1.95Å resolution

Crystal structure of the Patched-1 (PTCH1) ectodomain 1

Released:
DOI: 10.2210/pdb6rtx/pdb
PDB entry 6rtx coloured by chain, front view.
PDB entry 6rtx coloured by chain, side view.
PDB entry 6rtx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The morphogen Sonic hedgehog inhibits its receptor Patched by a pincer grasp mechanism.
Rudolf AF, Kinnebrew M, Kowatsch C, Ansell TB, El Omari K, Bishop B, Pardon E, Schwab RA, Malinauskas T, Qian M, Duman R, Covey DF, Steyaert J, Wagner A, Sansom MSP, Rohatgi R, Siebold C
Nat Chem Biol 15 975-982 (2019)
PMID: 31548691

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171795 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein patched homolog 1 Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.67 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q13635 (Residues: 139-428; Coverage: 20%)
Gene names: PTCH, PTCH1

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 49.17Å b: 65.68Å c: 100.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.257
Expression system: Homo sapiens

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Citations

14 review citations

Cholesterol access in cellular membranes controls Hedgehog signaling.
Radhakrishnan et al. (2020)
13 more