6ru1

X-ray diffraction
1.39Å resolution

Crystal Structure of Glucuronoyl Esterase from Cerrena unicolor inactive S270A variant in complex with the aldouronic acid Um4X

Released:

Function and Biology Details

Reaction catalysed:
A 4-O-methyl-D-glucopyranuronate ester + H(2)O = 4-O-methyl-D-glucuronic acid + an alcohol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-100829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
4-O-methyl-glucuronoyl methylesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 401 amino acids
Theoretical weight: 43.21 KDa
Source organism: Cerrena unicolor
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A0A7EQR3 (Residues: 95-474; Coverage: 83%)

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP, GCV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P41212
Unit cell:
a: 84.24Å b: 84.24Å c: 260.815Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.177
Expression system: Komagataella pastoris