6rv7

X-ray diffraction
1.73Å resolution

Crystal Structure of Glucuronoyl Esterase from Cerrena unicolor inactive S270A variant in complex with the aldouronic acid UXXR

Released:

Function and Biology Details

Reaction catalysed:
A 4-O-methyl-D-glucopyranuronate ester + H(2)O = 4-O-methyl-D-glucuronic acid + an alcohol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-100829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
4-O-methyl-glucuronoyl methylesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 401 amino acids
Theoretical weight: 43.21 KDa
Source organism: Cerrena unicolor
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A0A7EQR3 (Residues: 95-474; Coverage: 83%)

Ligands and Environments

Carbohydrate polymer : NEW Components: XYL, XYP, GCV
Carbohydrate polymer : NEW Components: XYP
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P41212
Unit cell:
a: 84.781Å b: 84.781Å c: 260.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.194
Expression system: Komagataella pastoris