› 6rvd

Electron Microscopy
3.5Å resolution

Revised cryo-EM structure of the human 2:1 Ptch1-Shh complex

Released:
DOI: 10.2210/pdb6rvd/pdb
PDB entry 6rvd coloured by chain, front view.
PDB entry 6rvd coloured by chain, side view.
PDB entry 6rvd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The morphogen Sonic hedgehog inhibits its receptor Patched by a pincer grasp mechanism.
Rudolf AF, Kinnebrew M, Kowatsch C, Ansell TB, El Omari K, Bishop B, Pardon E, Schwab RA, Malinauskas T, Qian M, Duman R, Covey DF, Steyaert J, Wagner A, Sansom MSP, Rohatgi R, Siebold C
Nat Chem Biol 15 975-982 (2019)
PMID: 31548691
Related structures: EMD-8955

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171800 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein patched homolog 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1447 amino acids
Theoretical weight: 160.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q13635 (Residues: 1-1447; Coverage: 100%)
Gene names: PTCH, PTCH1
Sequence domains: Patched family
Sonic hedgehog protein N-product Chain: C
Molecule details ›
Chain: C
Length: 174 amino acids
Theoretical weight: 19.59 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15465 (Residues: 24-197; Coverage: 40%)
Gene name: SHH
Sequence domains: Hedgehog amino-terminal signalling domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand CLR 4 x CLR
Ligand NAG 7 x NAG
Ligand PLM 1 x PLM
Ligand ZN 1 x ZN
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-8955
Expression system: Homo sapiens

Quick links

Citations

11 review citations

Structural and Functional Diversity of Resistance-Nodulation-Cell Division Transporters.
Klenotic et al. (2021)
10 more

9 mentions without citation

Ghrelin octanoylation by ghrelin O-acyltransferase: protein acylation impacting metabolic and neuroendocrine signalling.
Davis et al. (2021)
8 more