6rwy

Electron Microscopy
5.11Å resolution

Export apparatus core and inner rod of the Shigella type 3 secretion system

Released:
Source organism: Shigella flexneri
Primary publication:
Cryo-EM structure of the Shigella type III needle complex.
PLoS Pathog 16 e1008263 (2020)
PMID: 32092125
Related structures: EMD-10046

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 33-mer (preferred)
PDBe Complex ID:
PDB-CPX-141014 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Inner rod protein Chains: A, G, H, I, J, K
Molecule details ›
Chains: A, G, H, I, J, K
Length: 59 amino acids
Theoretical weight: 5.04 KDa
Source organism: Shigella flexneri
Inner rod protein Chains: B, C, D, E, F
Molecule details ›
Chains: B, C, D, E, F
Length: 73 amino acids
Theoretical weight: 6.23 KDa
Source organism: Shigella flexneri
Type 3 secretion system needle filament protein Chains: L, M, N, O, P, Q, R, S, T, U, V
Molecule details ›
Chains: L, M, N, O, P, Q, R, S, T, U, V
Length: 98 amino acids
Theoretical weight: 11.06 KDa
Source organism: Shigella flexneri
Expression system: Shigella flexneri
UniProt:
  • Canonical: P0A223 (Residues: 1-83; Coverage: 100%)
Gene names: CP0137, mxiH, sctF
Sequence domains: Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF
Surface presentation of antigens protein SpaP Chains: a, b, c, d, e
Molecule details ›
Chains: a, b, c, d, e
Length: 216 amino acids
Theoretical weight: 24.22 KDa
Source organism: Shigella flexneri
UniProt:
  • Canonical: P0A1L3 (Residues: 1-216; Coverage: 100%)
Gene names: CP0153, spa24, spaP
Sequence domains: FliP family
Surface presentation of antigens protein SpaR Chain: f
Molecule details ›
Chain: f
Length: 256 amino acids
Theoretical weight: 28.51 KDa
Source organism: Shigella flexneri
UniProt:
  • Canonical: P0A1M6 (Residues: 1-256; Coverage: 100%)
Gene names: CP0155, spa29, spaR
Sequence domains: Bacterial export proteins, family 1
Surface presentation of antigens protein SpaQ Chains: g, h, i, j, k
Molecule details ›
Chains: g, h, i, j, k
Length: 86 amino acids
Theoretical weight: 9.43 KDa
Source organism: Shigella flexneri
UniProt:
  • Canonical: P0A1M4 (Residues: 1-86; Coverage: 100%)
Gene names: CP0154, spa9, spaQ
Sequence domains: Bacterial export proteins, family 3

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.11Å
Relevant EMDB volumes: EMD-10046
Expression system: Shigella flexneri