› 6ryt

X-ray diffraction
2.1Å resolution

Engineered beta-lactoglobulin: variant M107L

Released:
DOI: 10.2210/pdb6ryt/pdb
PDB entry 6ryt coloured by chain, front view.
PDB entry 6ryt coloured by chain, side view.
PDB entry 6ryt coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure-based design approach to rational site-directed mutagenesis of β-lactoglobulin.
Bonarek P, Loch JI, Tworzydło M, Cooper DR, Milto K, Wróbel P, Kurpiewska K, Lewiński K
J Struct Biol 210 107493 (2020)
PMID: 32169624

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactoglobulin Chains: A, B
Molecule details ›
Chains: A, B
Length: 162 amino acids
Theoretical weight: 18.21 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02754 (Residues: 17-178; Coverage: 100%)
Gene name: LGB
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Unit cell:
a: 56.16Å b: 61.52Å c: 78.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.214 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Milk Processing Affects Structure, Bioavailability and Immunogenicity of β-lactoglobulin.
Broersen K. (2020)
4 other articles

1 mention without citation

Interactions of Whey Proteins with Metal Ions.
Rodzik et al. (2020)