› 6s1v

X-ray diffraction
1.64Å resolution

Crystal structure of dimeric M-PMV protease D26N mutant in complex with inhibitor

Released:
DOI: 10.2210/pdb6s1v/pdb
PDB entry 6s1v coloured by chain, front view.
PDB entry 6s1v coloured by chain, side view.
PDB entry 6s1v coloured by chain, top view.
Source organisms:
Primary publication:
Comparison of a retroviral protease in monomeric and dimeric states.
Wosicki S, Gilski M, Zabranska H, Pichova I, Jaskolski M
Acta Crystallogr D Struct Biol 75 904-917 (2019)
PMID: 31588922

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139638 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protease 13 kDa Chains: A, B
Molecule details ›
Chains: A, B
Length: 114 amino acids
Theoretical weight: 12.96 KDa
Source organism: Mason-Pfizer monkey virus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P07572 (Residues: 760-873; Coverage: 6%)
Gene name: gag-pro-pol
Sequence domains: Retroviral aspartyl protease
PRO-0A1-VAL-PSA-ALA-MET-THR Chain: I
Molecule details ›
Chain: I
Length: 7 amino acids
Theoretical weight: 886 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 0A1 1 x 0A1

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 49.947Å b: 29.504Å c: 85.39Å
α: 90° β: 101.71° γ: 90°
R-values:
R R work R free
0.18 0.179 0.22
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

Quick links

Citations

2 mentions without citation

Dimer Interface Organization is a Main Determinant of Intermonomeric Interactions and Correlates with Evolutionary Relationships of Retroviral and Retroviral-Like Ddi1 and Ddi2 Proteases.
Mótyán et al. (2020)
1 more