› 6s1w

X-ray diffraction
1.98Å resolution

Crystal structure of dimeric M-PMV protease D26N mutant

Released:
DOI: 10.2210/pdb6s1w/pdb
PDB entry 6s1w coloured by chain, front view.
PDB entry 6s1w coloured by chain, side view.
PDB entry 6s1w coloured by chain, top view.
Source organism: Mason-Pfizer monkey virus
Primary publication:
Comparison of a retroviral protease in monomeric and dimeric states.
Wosicki S, Gilski M, Zabranska H, Pichova I, Jaskolski M
Acta Crystallogr D Struct Biol 75 904-917 (2019)
PMID: 31588922

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139636 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protease 13 kDa Chains: A, B
Molecule details ›
Chains: A, B
Length: 114 amino acids
Theoretical weight: 12.96 KDa
Source organism: Mason-Pfizer monkey virus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P07572 (Residues: 760-873; Coverage: 6%)
Gene name: gag-pro-pol
Sequence domains: Retroviral aspartyl protease

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 49.987Å b: 29.571Å c: 85.2Å
α: 90° β: 102.52° γ: 90°
R-values:
R R work R free
0.203 0.2 0.261
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 mentions without citation

Dimer Interface Organization is a Main Determinant of Intermonomeric Interactions and Correlates with Evolutionary Relationships of Retroviral and Retroviral-Like Ddi1 and Ddi2 Proteases.
Mótyán et al. (2020)
1 more