› 6sbn

X-ray diffraction
1.09Å resolution

Polyester hydrolase PE-H of Pseudomonas aestusnigri

Released:
DOI: 10.2210/pdb6sbn/pdb
PDB entry 6sbn coloured by chain, front view.
PDB entry 6sbn coloured by chain, side view.
PDB entry 6sbn coloured by chain, top view.
Source organism: Halopseudomonas aestusnigri
Primary publication:
A Novel Polyester Hydrolase From the Marine Bacterium Pseudomonas aestusnigri - Structural and Functional Insights.
Bollinger A, Thies S, Knieps-Grünhagen E, Gertzen C, Kobus S, Höppner A, Ferrer M, Gohlke H, Smits SHJ, Jaeger KE
Front Microbiol 11 114 (2020)
PMID: 32117139

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103468 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dienelactone hydrolase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 33.41 KDa
Source organism: Halopseudomonas aestusnigri
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1H6AD45 (Residues: 1-304; Coverage: 100%)
Gene name: B7O88_11480
Sequence domains: Dienelactone hydrolase family

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 68.814Å b: 80.014Å c: 88.912Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.108 0.107 0.137
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Enzymatic Remediation of Polyethylene Terephthalate (PET)-Based Polymers for Effective Management of Plastic Wastes: An Overview.
Maurya et al. (2020)
15 more

8 mentions without citation

Microbial Polyethylene Terephthalate Hydrolases: Current and Future Perspectives.
Carr et al. (2020)
7 more