› 6sij

X-ray diffraction
1.61Å resolution

SAD structure of Hen Egg White Lysozyme recovered by continuous rotation data collection and multivariate analysis of Friedel pairs

Released:
DOI: 10.2210/pdb6sij/pdb
PDB entry 6sij coloured by chain, front view.
PDB entry 6sij coloured by chain, side view.
PDB entry 6sij coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Bayesian machine learning improves single-wavelength anomalous diffraction phasing.
Garcia-Bonete MJ, Katona G
Acta Crystallogr A Found Adv 75 851-860 (2019)
PMID: 31692460

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.26 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 1-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family

Ligands and Environments

4 bound ligands:
Ligand EDO 3 x EDO
Ligand ACT 2 x ACT
Ligand NA 1 x NA
Ligand CL 8 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P43212
Unit cell:
a: 79.07Å b: 79.07Å c: 36.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.141 0.167

Quick links

Citations

1 review citation

Emerging Time-Resolved X-Ray Diffraction Approaches for Protein Dynamics.
Hekstra DR. (2023)
6 other articles

1 mention without citation

Bayesian machine learning improves single-wavelength anomalous diffraction phasing.
Garcia-Bonete et al. (2019)