› 6sko

Electron Microscopy
3.4Å resolution

Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork - conformation 2 MCM CTD:ssDNA

Released:
DOI: 10.2210/pdb6sko/pdb
PDB entry 6sko coloured by chain, front view.
PDB entry 6sko coloured by chain, side view.
PDB entry 6sko coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork.
Baretić D, Jenkyn-Bedford M, Aria V, Cannone G, Skehel M, Yeeles JTP
Mol Cell 78 926-940.e13 (2020)
PMID: 32369734
Related structures: EMD-10230

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120616 (preferred)
Entry contents:
6 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (7 distinct):
DNA replication licensing factor MCM6 Chain: 6
Molecule details ›
Chain: 6
Length: 1017 amino acids
Theoretical weight: 113.11 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P53091 (Residues: 1-1017; Coverage: 100%)
Gene names: MCM6, YGL201C
Sequence domains:
DNA replication licensing factor MCM7 Chain: 7
Molecule details ›
Chain: 7
Length: 845 amino acids
Theoretical weight: 95.05 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38132 (Residues: 1-845; Coverage: 100%)
Gene names: CDC47, MCM7, YBR1441, YBR202W
Sequence domains:
DNA replication licensing factor MCM2 Chain: 2
Molecule details ›
Chain: 2
Length: 868 amino acids
Theoretical weight: 98.91 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29469 (Residues: 1-868; Coverage: 100%)
Gene names: MCM2, YBL023C, YBL0438
Sequence domains:
Minichromosome maintenance protein 5 Chain: 5
Molecule details ›
Chain: 5
Length: 775 amino acids
Theoretical weight: 86.51 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29496 (Residues: 1-775; Coverage: 100%)
Gene names: CDC46, L9328.1, MCM5, YLR274W
Sequence domains:
DNA replication licensing factor MCM3 Chain: 3
Molecule details ›
Chain: 3
Length: 971 amino acids
Theoretical weight: 107.65 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P24279 (Residues: 1-971; Coverage: 100%)
Gene names: MCM3, SYGP-ORF23, YEL032W
Sequence domains:
DNA replication licensing factor MCM4 Chain: 4
Molecule details ›
Chain: 4
Length: 933 amino acids
Theoretical weight: 105.14 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P30665 (Residues: 1-933; Coverage: 100%)
Gene names: CDC54, HCD21, MCM4, YP9531.13, YPR019W
Sequence domains:
ssDNA, leading-strand template Chain: I
Molecule details ›
Chain: I
Length: 85 nucleotides
Theoretical weight: 26.4 KDa

Ligands and Environments

2 bound ligands:
Ligand ANP 5 x ANP
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-10230
Expression system: Saccharomyces cerevisiae

Quick links

Citations

16 review citations

Present and Emerging Methodologies in Cryo-EM Single-Particle Analysis.
Wu et al. (2020)
15 more

2 mentions without citation

Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork.
Baretić et al. (2020)
1 more