› 6sun

X-ray diffraction
1.35Å resolution

Amicoumacin kinase hAmiN in complex with AMP-PNP, Ca2+ and Ami

Released:
DOI: 10.2210/pdb6sun/pdb
PDB entry 6sun coloured by chain, front view.
PDB entry 6sun coloured by chain, side view.
PDB entry 6sun coloured by chain, top view.
Source organism: Bacillus pumilus
Primary publication:
A kinase bioscavenger provides antibiotic resistance by extremely tight substrate binding.
Terekhov SS, Mokrushina YA, Nazarov AS, Zlobin A, Zalevsky A, Bourenkov G, Golovin A, Belogurov A, Osterman IA, Kulikova AA, Mitkevich VA, Lou HJ, Turk BE, Wilmanns M, Smirnov IV, Altman S, Gabibov AG
Sci Adv 6 eaaz9861 (2020)
PMID: 32637600

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167489 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
APH domain-containing protein, amicoumacin kinase Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 39.52 KDa
Source organism: Bacillus pumilus
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand ANP 1 x ANP
Ligand UAM 1 x UAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: C2
Unit cell:
a: 126.64Å b: 54.46Å c: 64.63Å
α: 90° β: 113.28° γ: 90°
R-values:
R R work R free
0.143 0.142 0.176
Expression system: Escherichia coli

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Citations

2 review citations

Overcoming Antibiotic Resistance with Novel Paradigms of Antibiotic Selection.
Tetz et al. (2022)
1 more